Services
Cell Culture Systems
CELL CULTURE SYSTEMS – HUMAN AND RODENT
- Primary human and rodent cell/tissue culture from multiple organ systems
- Long-term normal and transformed cell line culture
- Dose-response testing in cells to define drug activity or toxicity
- Generation of cell culture supernatants for growth factors, proteins, and matrix components
- Development of client-specific cell culture models for drug screening
PHARMACEUTICAL APPLICATIONS:
- Screening of compounds for activity or toxicity
- Defining human primary cell or cell line responses
- Supplementing of internal cell culture resources
- Mechanistic determinations of drug efficacy or toxicity
- Advanced cell culture approaches to mimic in vivo conditions
- Comparative testing of preclinical and clinical drug responses
- Cost-effective development new cell-based models
![Human Fibroblast](https://jvbiolabs.com/wp-content/uploads/2022/11/jvb1.jpg)
Human Skin Organ Culture
![Skin Organ Culture](https://jvbiolabs.com/wp-content/uploads/2022/11/jvb2.jpg)
– Multiple replicate cultures from each skin donor
– Viability and homeostasis maintained for weeks in culture
– Healthy skin, aged skin, photodamaged skin, psoriatic, diabetic skin etc.
– Can be used in conjunction with primary culture keratinocytes and fibroblasts from same donors
– Useful for early stage (pre-clinical) assessment of:
- Skin toxicity, corrosivity, contact irritation, contact sensitization
- Epidermal hyperplasia and skin thickening; Normal and abnormal differentiation and barrier function
- Skin atrophy / skin aging / prevention
- Dermal effects: Collagen, proteoglycan and glycoprotein production and degradation, MMPs, MMP activation and inhibition, other proteinases
- Fibroproliferative and fibrotic changes
- UV-skin damage
- Effects on hair follicles, sebaceous glands etc.
- Mast cell biology, vascular biology, melanocyte biology
- Skin immunology
– Organ-cultured skin useful for assessing cytokines, growth factors and other molecules of interest; Used for gene array and proteomic analysis; signaling pathway assessment
– Amenable to structural analysis: histology / cytology, immunohistology / immunofluorescence; and ultrastructure (TEM, SEM)
– Scientists at JV Biolabs have over 100 publications in use of this technology
human psoriatic skin – scid mouse transplant model
- The human psoriatic skin – scid mouse transplant model is the industry accepted model for preclinical assessment of potential anti-psoriatic agents.
- JV BIOLABS has over 10 years of experience with this model.
- Can assess clinical changes, histology features and immunohistology. Changes in growth factor / cytokine production with treatment.
- Topical, systemic and oral dosing is possible.
- Transplantation model can be used alone for demonstration of therapeutic efficacy.
- Transplantation model can be used in conjunction with human skin organ culture and keratinocyte culture to assess mechanisms of action.
![](https://jvbiolabs.com/wp-content/uploads/2022/11/jvb3.jpg)
Rhinomouse Model
![Rhinomouse Model](https://jvbiolabs.com/wp-content/uploads/2022/11/jvb4.jpg)
THE RHINOMOUSE MODEL IS THE INDUSTRY ACCEPTED MODEL FOR PRECLINICAL ASSESSMENT OF RETINOIDS FOR ANTI-ACNE ACTIVITY
- JV Biolabs has over 15 years of experience with this model
- Assess efficacy (anti-keratinization), inflammation, skin irritation, systemic toxicity in same
- experiment
- Topical, systemic and oral dosing possible
- Native molecules and formulated products can be employed
- Can be used alone for demonstration of therapeutic efficacy
- Can be used in conjunction with human skin organ culture and keratinocyte culture to assess
- mechanisms of action
- Non-retinoids compounds can also be evaluated in the rhinomouse model
Vascular Biology and Endothelial Cell Models
SYSTEMS FOR ENDOTHELIAL BIOLOGY
- Acute and chronic vascular injury models
- Human primary endothelial cells and established cell lines
- Healthy volunteer, psoriatic, Rosacea, cancer, and fibrotic systems
- Cytokine, integrin, and cellsurface marker characterization of vessels or individual cells
- Rodent, bovine and pocine endothelial cells and tissues
PHARMACEUTICAL APPLICATIONS
- Direct and indirect drug-mediated injury
- Localized injection site responses
- Drug compatibility and irritation assessment
- Mechanistic determinations of vascular changes
- Apoptotic and anti-apoptotic mechanisms
- Assessment of angiogenic and anti-angiogenic activity
- Drug-induced vascular toxicity
![Vascular Damage](https://jvbiolabs.com/wp-content/uploads/2022/11/jvb6.jpg)
Kidney Injury Models
![Kidney Injury](https://jvbiolabs.com/wp-content/uploads/2022/11/jvb7.jpg)
SYSTEMS FOR DETECTING RENAL INJURY AND FUNCTION
- In vivo models of drug-induced nephrotoxicity, diabetes, vasculitis, and glomerulonephritis
- In vivo models of vasculitis and imflammatory injury
- In vivo characterization of glomerular microangiopathic injury
- Human and rodent podocyte culture – primary and cloned cells
- Mesangial and tubular cell culture
- ELISA and standard clinical pathology monitoring of renal biomarkers
PHARMACEUTICAL APPLICATIONS
- Detection of drug-induced changes on kidney and kidney function
- Screening of drug candidates for kidney toxicity
- Direct and indirect drug-mediated injury characterization
- Mechanistic determinations of pharmacologic activity
- Specific characterization of kidney injury pathways
- Therapeutic potential of new therapies – small molecule and biologic
Human, Cynomologus Monkey, and Rodent Mast Cell Assays
IN VITRO CHALLENGE OF ISOLATED MAST CELLS
- Rat Peritoneal Mast Cells
- Human Mast Cell Line
- Human Peripheral Blood Granulocytes
- Cynomolgous Monkey Peripheral Blood Granulocytes
DETERMINATIONS OF MAST CELL DEGRANULATION
HUMAN & CYNOMOLOGUS MONKEYS
- Histamine
- Tryptase
- Beta-Hexosaminidase
- Toxicity as measured by LDH
RAT MAST CELLS
- Histamine
- Tryptase
- Chymase
- Toxicity as measured by LDH
PHARMACEUTICAL APPLICATIONS
- Screening of compounds for hypersensitivity potential
- Screening of compounds for mast cell activation
- Suppression of mast cell activation
![](https://jvbiolabs.com/wp-content/uploads/2022/11/jvb8.jpg)
![](https://jvbiolabs.com/wp-content/uploads/2022/11/jvb9-150x150.jpg)
Human And Animal Models For Mast Cell Biology
![Mast Cells](https://jvbiolabs.com/wp-content/uploads/2022/11/jvb10.jpg)
HUMAN AND RODENT MAST CELL SYSTEMS
- Human skin organ culture to assess mast cell numbers and degranulation
- In vitro challenge of rat peritoneal mast cells for histamine and/or IL-17 release
- Measurement of histamine, chymase, tryptase, and IL-17 release
- Histology and cytology using immunohistochemistry and ultrastructure methods
- Development of client-specific applications for drug screening
PHARMACEUTICAL APPLICATIONS
- Screening of compounds for immediate hypersensitivity potential
- Supplementing of internal testing resources
- Mechanistic determinations of drug efficacy or toxicity
- Suppression of mast cell activation
- Comparative testing of preclinical and clinical drug responses
Proteomic Micro-Arrays
SYSTEMS FOR PLASMA, SERUM, AND CELL CULTURE SUPERNATANTS
Rapid and specific measurement of:
- Cytokines and chemokines
- Inflammatory proteins
- Growth and angiogenesis factors
- Protease and protease inhibitors
- Cell adhesion molecules
- Heat shock proteins
- Complement proteins
- Anti-coagulant factors
- >100 Human proteins and >45 rat proteins detected on the standard antibody array with low pg detections
- Custom microarray development
- High sample throughput with semiquantitation evaluation of multiple individual proteins
PHARMACEUTICAL APPLICATIONS
- Analysis of clinical and preclinical samples for biomarker development
- Detection of auto-antibodies
- Direct and indirect drug-mediated injury characterization
- Mechanistic determinations of pharmacologic and toxicologic activity
- Sample screening to determine which proteins to target in renal disease,
- inflammatory reactions, auto-immunity, and transplantation
![High Throughput Micro-Arrays](https://jvbiolabs.com/wp-content/uploads/2022/11/jvb11.jpg)
Research ELISA Development And Conduct
![Standard ELISA System](https://jvbiolabs.com/wp-content/uploads/2022/11/jvb12.jpg)
ELISA OFFERINGS
- Accurate and reproducible performance of commercial or client ELISA assays
- Development of new research ELISA methods to client’s performance specifications
- Analysis of human, animal, and cell culture samples
- Extensive menu of available cytokine, protease, peptide, and growth factor assays
- Generation of positive control reagents and reference materials
PHARMACEUTICAL APPLICATIONS
- Characterization of drug-induced effects from in vitro and in vivo models
- Cost-effective assay development for early-stage applications
- Mechanistic study support
- Supplementing of internal resources for sensitive ELISA methods
- Exploratory investigations of clinical specimens
- Screening immunogenicity determinations
- Third-part validation of test results
Tumor Immunology
FULL RANGE OF MODELS FOR STUDY OF TUMOR IMMUNOLOGY
- Human tumors in xenograft models
- Rodent tumors in syngeneic host
- Carcinogenesis / anti-carcinogenesis models
- 2D and 3D cell culture
PHARMACEUTICAL APPLICATIONS
- Preclinical assessment in support of Mab and vaccine development programs
- Detection and quantification of target molecules on tumor cells and normal counterparts by confocal immunofluoresce microscopy, flow cytometry, immunohistology, ELISA and western blotting
- Assessment of efficacy with monoclonal antibodies, peptides and immune modulators in cell culture models, in syngeneic rodent models and in human xenograft models
- Studies to assess mechanisms of action
- Target molecule modulati
![](https://jvbiolabs.com/wp-content/uploads/2022/11/jvb13.jpg)
Tumor Biology
![Tumor Biology](https://jvbiolabs.com/wp-content/uploads/2022/11/jvb14.jpg)
FULL RANGE OF MODEL SYSTEMS OR STUDY OF TUMOR CELL BIOLOGY
- Human tissue – normal, premalignant and malignant in culture
- Xenograft models
- Rodent tumor models for primary tumor and metastatic growth
- 2D and 3D cell culture
PHARMACEUTICAL APPLICATIONS
- Preclinical assessment of therapeutic efficacy in cell culture models and in ex vivo human tissue models
- Dietary / therapeutic intervention in long-term rodent models
- Mechanisms of action with small molecule inhibitors and Mabs
- Models for carcinogenesis and anti-carcinogenesis
- Biomarker detection and modulation